SART3 antibody (C-Term)

Catalog No. ABIN374289

The Goat Polyclonal anti-SART3 antibody (ABIN374289) specifically detects SART3 in WB. The antibody is reactive with Human samples.

Quick Overview for SART3 antibody (C-Term) (ABIN374289)

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SART3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SART3 Antibody

Binding Specificity: C-Term

Sequence: C-MSNADFAKLF LRK

Specificity: This antibody detects SART3 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_055521.1 Genename: SART3

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.01-0.03 μg/mL. Approx 170 kDa band observed in lysates of cell lines Daudi,Jurkat and MOLT4 (calculated MW of 110 kDa according to NP_055521.1). The observedmolecular weight corresponds to earlier findings in literature with different antibodies(Yang et al, Cancer Res.1999 Aug 15,59(16): 4056-63, PMID: 10463607).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SART3

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Alternative Name: SART3 / TIP110

Background: Synonyms: KIAA0156, SART-3, Squamous cell carcinoma antigen recognized by T-cells 3, Tat-interacting protein of 110 kDa

Gene ID: 9733

NCBI Accession: NP_055521

UniProt: Q15020

Pathways: Ribonucleoprotein Complex Subunit Organization