Ube2t antibody (C-Term)

Catalog No. ABIN374292

This anti-Ube2t antibody is a Goat Polyclonal antibody detecting Ube2t in WB. Suitable for Human.

Quick Overview for Ube2t antibody (C-Term) (ABIN374292)

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Ube2t antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Ube2t Antibody

Binding Specificity: C-Term

Sequence: C-QLVGIEKKFH PDV

Specificity: This antibody detects UBE2T at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_054895.1. Genename: UBE2T

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.1-0.3 μg/mL. Approx.22 kDa band observed in lysates of cell lines K562,HEK293 and A549 (calculated MW of 22.2 kDa according to NP_054895.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Ube2t

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Alternative Name: UBE2T

Background: Synonyms: HSPC150, Ubiquitin carrier protein T, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T

Gene ID: 29089

NCBI Accession: NP_054895

UniProt: Q9NPD8

Pathways: Chromatin Binding