STXBP6 antibody (N-Term)

Catalog No. ABIN374321

The Goat Polyclonal anti-STXBP6 antibody is suitable to detect STXBP6 in samples from Human, Mouse, Rat and Cow. It has been validated for WB and IHC (p).

Quick Overview for STXBP6 antibody (N-Term) (ABIN374321)

Target: STXBP6 (Syntaxin Binding Protein 6 (Amisyn) (STXBP6))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This STXBP6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STXBP6 Antibody

Binding Specificity: N-Term

Sequence: SAKSAISKEI FAP-C

Specificity: This antibody detects STXBP6 / Amysin at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Cow and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from N Terminus of the protein sequence according to NP_054897.4. Genename: STXBP6

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: Experiments gave bands at both approx 25 kDa and approx 45 kDa in HumanBrain extracts at 0.1 μg/mL. Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Kidney shows cytoplasmstaining of PCT and DCT cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for STXBP6

Target: STXBP6 (Syntaxin Binding Protein 6 (Amisyn) (STXBP6))

Background: Amisyn forms non-fusogenic complexes with SNAP25 and STX1A and may thereby modulate the formation of functional SNARE complexes and exocytosis. It is detected at low levels in brain and at very low levels in heart, adrenal gland, testis, liver and kidney. There are three named isoforms produced by alternative splicing.Synonyms: HSPC156, Syntaxin-binding protein 6

Gene ID: 29091

NCBI Accession: NP_054897

UniProt: Q8NFX7