PES1 antibody (N-Term)

Catalog No. ABIN374331

The Goat Polyclonal anti-PES1 antibody (ABIN374331) specifically detects PES1 in WB, IHC (p) and EIA. The antibody is reactive with Human, Mouse, Cow and Pig samples.

Quick Overview for PES1 antibody (N-Term) (ABIN374331)

Target: PES1 (Pescadillo Ribosomal Biogenesis Factor 1 (PES1))

Reactivity: Human, Mouse, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PES1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PES1 Antibody

Binding Specificity: N-Term

Sequence: GGLEKKKYER GSA-C

Specificity: This antibody detects Pescadillo homolog (PES1) at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptid from N Terminus of the protein sequence according to NP_055118.1. Genename: PES1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 70 kDa band observed in HeLa lysate (calculated MW of60 kDa according to NP_055118).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PES1

Target: PES1 (Pescadillo Ribosomal Biogenesis Factor 1 (PES1))

Alternative Name: Pescadillo Homolog (PES1)

Background: Pescadillo may play a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression. The protein is abnormally elevated in malignant tumors of astrocytic origin. It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product.

Gene ID: 23481

NCBI Accession: NP_055118

UniProt: O00541