LMO7 antibody (Internal Region)

Catalog No. ABIN374384

The Goat Polyclonal anti-LMO7 antibody is suitable to detect LMO7 in samples from Human. It has been validated for WB.

Quick Overview for LMO7 antibody (Internal Region) (ABIN374384)

Target: LMO7 (LIM Domain 7 (LMO7))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LMO7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LMO7 Antibody

Binding Specificity: Internal Region

Sequence: ESTCVSASLP RS

Specificity: This antibody detects internal LMO7.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to AAD33924.1 Genename: LMO7

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 70-75 kDa band and approx 35 kDa band observed inHuman Lung lysates (predicted MWs of 95 kDa according to AAD33924.1 - however ourresult agrees with Putilina et al as below). A minor band of unknown identity was alsoconsistently observed at approx 55 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for LMO7

Target: LMO7 (LIM Domain 7 (LMO7))

Alternative Name: LMO7

Background: LMO7 contains a calponin homology (CH) domain, a PDZ domain, and a LIM domain. An F-box (FBX) domain is present in alternative splice variants. Members of the LIM protein family carry the LIM domain, a unique cysteine-rich zinc-binding domain. Members of the FBX protein family are involved in protein-protein interactions. LMO7 may be involved in protein-protein interactions. Multiple alternative splice variants have been described but their full-length sequences have not been determined.Synonyms: F-box only protein 20, FBX20, FBXO20, KIAA0858, LIM domain only protein 7, LMO7, LOMP

Gene ID: 4008

UniProt: Q5TBK6