RANGAP1 antibody (N-Term)

Catalog No. ABIN374398

This Goat Polyclonal antibody specifically detects RANGAP1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Dog and Cow.

Quick Overview for RANGAP1 antibody (N-Term) (ABIN374398)

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This RANGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RANGAP1 Antibody

Binding Specificity: N-Term

Sequence: ASEDIAKLAE TLAK-C

Specificity: This antibody detects RANGAP1 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Dog, Pig.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_002874.1 Genename: RANGAP1

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: Approx 70 kDa band observed in Human Brain and 3T3 lysates (predicted MWof 60 kDa according to NP_002874). Recommended concentration: 0.1 - 0.5 μg/mL. Immunohistochemistry on paraffin sections: Human Liver shows vesicular staining in thecytoplasm of hepatocytes. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RANGAP1

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Alternative Name: RanGAP1

Background: Synonyms: KIAA1835, Ran GTPase-activating protein 1, SD, segregation distorter homolog

Gene ID: 5905

NCBI Accession: NP_002874

UniProt: P46060

Pathways: M Phase, Protein targeting to Nucleus