VPS28 antibody (C-Term)

Catalog No. ABIN374405

Product Details anti-VPS28 Antibody

Target: VPS28 (Vacuolar Protein Sorting 28 (VPS28))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS28 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-ESAYNAFNRF LHA

Specificity: This antibody detects VPS28 isoform 1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, PigSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_057292.1 Genename: VPS28

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 1000. Western blot: 1-3 μg/mL. Approx 24 kDa band observed in H460, Human Testis and Brainlysates (predicted MW of 24 kDa according to NP_057292.1). Immunohistochemistry: 10 μg/mL. In paraffin embedded Human Testis, stains acrosomalhead caps in round spermatids.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for VPS28

Target: VPS28 (Vacuolar Protein Sorting 28 (VPS28))

Alternative Name: VPS28 (VPS28 Products)

Background: Synonyms: ESCRT-I complex subunit VPS28, Vacuolar protein sorting-associated protein 28 homolog

Gene ID: 51160

NCBI Accession: NP_057292

UniProt: Q9UK41