GAPDH antibody (C-Term)

Catalog No. ABIN374435

The Goat Polyclonal anti-GAPDH antibody has been validated for WB, IF, IHC (p) and EIA. It is suitable to detect GAPDH in samples from Human, Mouse, Rat and Dog. There are 10+ publications available.

Quick Overview for GAPDH antibody (C-Term) (ABIN374435)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GAPDH Antibody

Binding Specificity: C-Term

Sequence: C-HQVVSSDFNS DT

Specificity: This antibody is specific to GAPDH at C-Term. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a Loading/Positive Control is required in Western blotting.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_002037.2.

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/2000Western Blot: 0.01-0.03 μg/mL. A approx. 35 kDa band is observed in lysates of Human Tonsil,MouseSpleen, and cell lines HeLa, A431 and 293. Immunohistochemistry In Paraffin Embedded Tonsil: 0.3 μg/mL. Staining of cells ingerminal centre, mantle zone, interfollicular area, epithelium and endothelium is

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-GAPDH antibody (ABIN374435)

Farris, Pifer, Zheng, Gottlieb, Denvir, Frisch: "Grainyhead-like 2 Reverses the Metabolic Changes Induced by the Oncogenic Epithelial-Mesenchymal Transition: Effects on Anoikis." in: Molecular cancer research : MCR, Vol. 14, Issue 6, pp. 528-38, (2017) (PubMed).

Breitenbach, Gruber, Klausegger, Trost, Bogner, Reitsamer, Bauer: "Pseudosyndactyly - an inflammatory and fibrotic wound healing disorder in recessive dystrophic epidermolysis bullosa." in: Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG, Vol. 13, Issue 12, pp. 1257-66, (2016) (PubMed).

Herzog, Rid, Wagner, Hundsberger, Eger, Bauer, Önder: "Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways." in: BMC research notes, Vol. 8, pp. 785, (2016) (PubMed).

Happold, Roth, Silginer, Florea, Lamszus, Frei, Deenen, Reifenberger, Weller: "Interferon-? induces loss of spherogenicity and overcomes therapy resistance of glioblastoma stem cells." in: Molecular cancer therapeutics, Vol. 13, Issue 4, pp. 948-61, (2014) (PubMed).

Xu, Weerachayaphorn, Cai, Soroka, Boyer: "Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 1, pp. G126-35, (2010) (PubMed).

Kiepe, Van Der Pas, Ciarmatori, Ständker, Schütt, Hoeflich, Hügel, Oh, Tönshoff: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." in: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, (2008) (PubMed).

Gerstner, Bremer, Vander Heyden, Lavaute, Yin, Landry: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." in: PLoS ONE, Vol. 3, Issue 2, pp. e1631, (2008) (PubMed).

Henson, Roberts, Salimi, Vadlamudi, Hamer, Gilmore, Jarskog, Philpot: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, (2008) (PubMed).

Nguyen, Pace, Koller: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." in: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, (2007) (PubMed).

Hagiwara, Tajika, Matsuzaki, Suzuki, Aoki, Takata: "Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome." in: Histochemistry and cell biology, Vol. 126, Issue 2, pp. 251-9, (2006) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme originally thought to be only involved in Glycolysis. GAPDH is constitutively expressed at high levels in almost all tissues and serves therefore as a useful marker for the measurement of equal loading in western blotting experiments. Because of its ubiquitous expression, GAPDH is often seen as a housekeeping gene. However in the last two decades, every year a new function for this enzyme has been revealed. Besides its central role as a cytoplasmic enzyme in Glycolysis, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as DNA replication and repair, nuclear membrane fusion, endocytosis, nuclear translocation in apoptosis, microtubule bundling, vesicular secretory transport, maintenance of telomere structure, phosphotransferase activity and nuclear RNA export. We now know that GAPDH is not only in the cytoplasm, but it shuttles between the cytoplasm and the nucleus and membranes. A lot of reports demonstrate the role of GAPDH in different pathologies such as progression in prostate cancer and ovarian cancer (mRNA stability), programmed neuronal cell death, and agerelated neuronal diseases. For example the sub-cellular distribution of GAPDH is abnormal in patients with Alzheimer's disease.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase

Molecular Weight: 36.1 kDa (Calculated)

Gene ID: 2597

NCBI Accession: NP_002037

UniProt: P04406