PD-L1 antibody (C-Term)

Catalog No. ABIN374512

This Goat Polyclonal antibody specifically detects PD-L1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PD-L1 antibody (C-Term) (ABIN374512)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PD-L1 Antibody

Binding Specificity: C-Term

Sequence: CKKQSDTHLE ET

Specificity: This antibody recognizes Human CD274 / PDL1. Other species not tested.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the C-Terminus of Human (NP_054862.1) Genename: CD274

Application Details

Application Notes: Peptide ELISA: 1/8000. Western blot: 0.3-1.0 μg/mL. Approx 35 kDa band observed in Human Heart lysates(calculated MW of 33.5 kDa according to NP_054862.1). Immunohistochemistry: 4-6 μg/mL. In paraffin embedded Human Skeletal Muscle shows staining of the striated muscularfibres.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274 / PDL1

Background: CD274 [PDL1] is expressed by antigen presenting cells, including human peripheral blood monocytes stimulated with interferon gamma, and activated human and murine dendritic cells. In addition, PDL1 is expressed in nonlymphoid tissues such as heart and lung. The relative levels of inhibitory PDL1 and costimulatory B7 1/B7 2 signals on antigen presenting cells may determine the extent of T cell activation and consequently the threshold between tolerance and autoimmunity. PDL1 expression on nonlymphoid tissues and its potential interaction with PD1 may subsequently determine the extent of immune responses at sites of inflammation. PDL1 is thought to be involved in fetomaternal tolerance.Synonyms: B7 homolog 1, B7H1, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, Programmed death ligand 1

Gene ID: 29126, 9606

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints