APOE antibody (C-Term)

Catalog No. ABIN374539

This Goat Polyclonal antibody specifically detects APOE in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for APOE antibody (C-Term) (ABIN374539)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-APOE Antibody

Binding Specificity: C-Term

Sequence: C-VGTSAAPVPS DNH

Specificity: This antibody recognizes Apolipoprotein E (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_000032.1. Genename: APOE

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: Apolipoprotein E (Apo E)

Background: Apolipoprotein E is essential for the normal catabolism of triglyceride rich lipoprotein constituents. The apolipoprotein E gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in Apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Mutations in the APOE gene confer susceptibility to Alzheimer's disease by affecting amyloid-beta deposition.Synonyms: Apo-E, ApoE

Gene ID: 348, 9606

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism