PVALB antibody (C-Term)

Catalog No. ABIN374572

This Goat Polyclonal antibody specifically detects PVALB in WB and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for PVALB antibody (C-Term) (ABIN374572)

Target: PVALB (Parvalbumin (PVALB))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PVALB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PVALB Antibody

Binding Specificity: C-Term

Sequence: C-GVDEFSTLVA ES

Specificity: This antibody recognizes Parvalbumin.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_002845.1.

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx 12 kDa band observed in Human Brain lysates (calculated MW of 12.2 kDa accordingto NP_002845.1). Approx 10-12 kDa band observed in rat skeletal muscle and in rat heart cells transfectedwith human PVALB, but not in untransfected rat heart cells. (1)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PVALB

Target: PVALB (Parvalbumin (PVALB))

Alternative Name: Parvalbumin alpha / PVALB

Background: Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.

Gene ID: 5816

NCBI Accession: NP_002845

UniProt: P20472