Clusterin antibody (C-Term)

Catalog No. ABIN374606

The Goat Polyclonal anti-Clusterin antibody is suitable to detect Clusterin in samples from Human and Dog. It has been validated for WB.

Quick Overview for Clusterin antibody (C-Term) (ABIN374606)

Target: Clusterin (CLU)

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Clusterin Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to Apolipoprotein J (Apo J).

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-EKALQEYRKKHREE, from the C Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.3 - 1.0 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Apolipoprotein J (Apo J)

Background: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

Gene ID: 1191, 9606

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling