Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody recognizes Human Chromobox protein homolog 5 (CBX5).
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Rat. Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen
Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1, NP_001120793.1, NP_001120794.1. .Remarks: Variants (NP_036249.1, NP_001120793.1, NP_001120794.1) encode the same protein
CBX5
Reactivity: Human
WB, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: 1/128000. Western Blot: 0.03 - 0.1 μg/mL. Immunohistochemistry on paraffin sections: 2 - 3 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are required for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin.Synonyms: Chromobox protein homolog 5, HP1 alpha, HP1-alpha, HP1A, Heterochromatin protein 1 homolog alpha, p25