PDE4D antibody (C-Term)

Catalog No. ABIN374635

This Goat Polyclonal antibody specifically detects PDE4D in WB and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PDE4D antibody (C-Term) (ABIN374635)

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PDE4D antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PDE4D Antibody

Binding Specificity: C-Term

Sequence: QPEACVIDDR SPDT

Specificity: This antibody detects PDE4D at C-term and is expected to recognize all reported isoforms.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_001098101.1, NP_006194.2, NP_001159371.1. Genename: PDE4D

Application Details

Application Notes: Peptide ELISA: 1/128000. Western Blot. : 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PDE4D

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Alternative Name: PDE4D

Background: Cyclic nucleotide phosphodiesterases (PDEs) catalyse the hydrolytic inactivation of the common intracellular second messengers cyclic adenosine and guanosine 3', 5'- monophosphate (cAMP and cGMP). Thus, these enzymes play a critical role in the regulation of a wide range of physiological processes modulated by cyclic nucleotide signalling. The PDE4 enzyme belongs to a family of cAMP-dependent PDEs that provide the major means of inactivating the key intracellular second messenger, cAMP. Four genes (4A, 4B, 4C, and 4D) encode around 20 distinct isoform members of the PDE4 family. Each isoform is characterized by a unique N-terminal region.Synonyms: 5'-cyclic phosphodiesterase 4D, DPDE3, EC=3.1.4.17, PDE43, PDE4D, cAMP-specific 3'

Gene ID: 5144

NCBI Accession: NP_001098101

UniProt: Q08499

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling