AIRE antibody (Internal Region, Isoform 1)

Catalog No. ABIN374692

This Goat Polyclonal antibody specifically detects AIRE in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Rat and Dog. It has been mentioned in 6+ publications

Quick Overview for AIRE antibody (Internal Region, Isoform 1) (ABIN374692)

Target: AIRE (Autoimmune Regulator (AIRE))

Reactivity: Human, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-AIRE Antibody

Binding Specificity: Internal Region, Isoform 1

Sequence: C-KDVDLSQPRK GRKP

Specificity: This antibody is expected to recognise isoform 1 (NP_000374.1) only.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine and Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000374.1.

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: 0.5-1.0 μg/mL. Immunohistochemistry: 2.5 μg/mL. In paraffin embedded Human Thymus shows nuclear staining in select thymocytes.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

References for anti-AIRE antibody (ABIN374692)

Nakasatomi, Maeshima, Mishima, Ikeuchi, Sakairi, Kaneko, Hiromura, Nojima: "Novel approach for the detection of tubular cell migration into the interstitium during renal fibrosis in rats." in: Fibrogenesis & tissue repair, Vol. 8, pp. 12, (2015) (PubMed).

Ströbel, Hartmann, Rosenwald, Kalla, Ott, Friedel, Schalke, Kasahara, Tomaru, Marx: "Corticomedullary differentiation and maturational arrest in thymomas." in: Histopathology, Vol. 64, Issue 4, pp. 557-66, (2014) (PubMed).

Jüngling, Liu, Lesting, Coulon, Sosulina, Reinscheid, Pape: "Activation of neuropeptide S-expressing neurons in the locus coeruleus by corticotropin-releasing factor." in: The Journal of physiology, Vol. 590, Issue Pt 16, pp. 3701-17, (2012) (PubMed).

Wei, Akerman, Newey, Pan, Clinch, Jacob, Shen, Wilkins, Ellory: "The potassium-chloride cotransporter 2 promotes cervical cancer cell migration and invasion by an ion transport-independent mechanism." in: The Journal of physiology, Vol. 589, Issue Pt 22, pp. 5349-59, (2011) (PubMed).

Yuan, Zhu, Zhou, Chen, Zhu, Ma, He, Tian, Shi: "Role of mast cell activation in inducing microglial cells to release neurotrophin." in: Journal of neuroscience research, Vol. 88, Issue 6, pp. 1348-54, (2010) (PubMed).

Zelano, Berg, Thams, Hailer, Cullheim: "SynCAM1 expression correlates with restoration of central synapses on spinal motoneurons after two different models of peripheral nerve injury." in: The Journal of comparative neurology, Vol. 517, Issue 5, pp. 670-82, (2009) (PubMed).

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE / APECED

Background: AIRE is a transcriptional regulator that is thought to be important in controlling tissue-specific expression of genes in the thymus. The precise function of the protein is not well defined. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator

Gene ID: 326, 9606

UniProt: O43918

Pathways: Chromatin Binding