Ezrin antibody (Internal Region)

Catalog No. ABIN374707

This Goat Polyclonal antibody specifically detects Ezrin in WB, IHC (p) and EIA. It exhibits reactivity toward Human and Dog.

Quick Overview for Ezrin antibody (Internal Region) (ABIN374707)

Target: Ezrin (EZR)

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Ezrin Antibody

Binding Specificity: Internal Region

Sequence: C-QDYEEKTKKA ER

Specificity: This antibody reacts to Ezrin / villin 2.

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003370.2. Genename: VIL2

Application Details

Application Notes: ELISA: 1/16000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background: Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.Synonyms: Cytovillin, EZR, VIL2, Villin-2, p81

Gene ID: 7430, 9606

UniProt: P15311

Pathways: Maintenance of Protein Location