GPX4 antibody (C-Term, Isoform A)

Catalog No. ABIN374711

The Goat Polyclonal anti-GPX4 antibody is suitable to detect GPX4 in samples from Human, Mouse, Rat, Cow and Pig. It has been validated for WB, IHC (p) and EIA.

Quick Overview for GPX4 antibody (C-Term, Isoform A) (ABIN374711)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GPX4 Antibody

Binding Specificity: C-Term, Isoform A

Specificity: This antibody is expected to recognize isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Pig, Cow.
Species reactivity (tested):Human.

Purification: Affinity Chromatpography

Immunogen: Peptide with sequence (C-EEPLVIEKDLPHY) from the C-terminus of the protein sequencen (NP_002076.2, NP_001034937.1).

Application Details

Application Notes: ELISA: 1/16000. Western Blot: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: Glutathione Peroxidase 4 / GPX4

Background: Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.Synonyms: GPx-4, Phospholipid hydroperoxide glutathione peroxidase mitochondrial

Gene ID: 2879

NCBI Accession: NP_001034936

UniProt: P36969