CLIC4 antibody (N-Term)

Catalog No. ABIN374756

The Goat Polyclonal anti-CLIC4 antibody has been validated for WB and IF. It is suitable to detect CLIC4 in samples from Human, Mouse, Rat and Dog.

Quick Overview for CLIC4 antibody (N-Term) (ABIN374756)

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This CLIC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-CLIC4 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to CLIC4.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine.
Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Immunogen: Peptide with sequence NGLKEEDKEPLIE-C, from the N Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.1 - 0.3 μg/mL. Immunofluorescence: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CLIC4

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Alternative Name: CLIC4

Background: Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH , and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family, the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells) (from Entrez Gene). CLIC4 is thought to play a role in apoptosis and has been shown to translocate to the nucleus under stress conditions.Synonyms: Chloride Intracellular Channel Protein 4, Intracellular Chloride Ion Channel Protein p64H1, mc3s5, mtCLIC, p64H1

Gene ID: 25932

NCBI Accession: NP_039234

UniProt: Q9Y696