SALL4 antibody (Internal Region)

Catalog No. ABIN374800

This Goat Polyclonal antibody specifically detects SALL4 in WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for SALL4 antibody (Internal Region) (ABIN374800)

Target: SALL4 (Sal-Like 4 (SALL4))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SALL4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SALL4 Antibody

Binding Specificity: Internal Region

Sequence: C-DQSKKGKGKP QN

Specificity: This antibody detects SALL4.

Cross-Reactivity (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Rat

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_780512.2, NP_958797.2, NP_958798.2. Genename: Sall4

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 70 kDa band observed in Rat Spleen and Testis lysates(calculated MW of 66.0 kDa according to NP_958797.2). Additional bands of unknownidentity was also consistently observed at 28+20 kDa. These bands were successfullyblocked by incubation with the immunising peptide. We would appreciate any feedbackfrom people in the field - have any such results been reported with otherantibodies/lysates? Have any further splice variants/modified forms been reported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SALL4

Target: SALL4 (Sal-Like 4 (SALL4))

Alternative Name: Sal-Like Protein 4 (SALL4)

Background: Sall4 is a probable transcription factor. Defects in Sall4 are the cause of Okihiro syndrome, also known as Duane radial ray syndrome (DRRS). It is a disorder characterized by the association of forearm malformations with Duane retraction syndrome. Sall4 is also involved in forelimb and heart development in miceSynonyms: Zinc finger protein SALL4

Gene ID: 99377

NCBI Accession: NP_780512

UniProt: Q8BX22

Pathways: Stem Cell Maintenance, Tube Formation