ASS1 antibody (Internal Region)

Catalog No. ABIN374809

This Goat Polyclonal antibody specifically detects ASS1 in WB, IF, IP, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow and Dog.

Quick Overview for ASS1 antibody (Internal Region) (ABIN374809)

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ASS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ASS1 Antibody

Binding Specificity: Internal Region

Sequence: C-ENPKNQAPPG LYTKTQD

Specificity: This antibody detects ASS1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human, Cow.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000041.2, NP_446464.1. Genename: ASS1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: Approx 45 kDa band observed in Human Kidney lysates (calculated MW of46.5 kDa according to NP_000041.2 and NP_446464.1). Recommended concentration: 0.01-0.03 μg/mL. Immunofluorescence: A customer has reported this antibody works well in IF. Immunoprecipitation: This antibody has been successfully used in immunoprecipitationassays (Corbin et al. , 2008).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ASS1

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Alternative Name: ASS1

Background: ASS1 belongs to the argininosuccinate synthase family, type 1 subfamily. It catalyzes the penultimate step of the arginine biosynthetic pathway. Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700].Synonyms: Argininosuccinate synthase, Citrulline-aspartate ligase

Molecular Weight: 46.5 kDa (NP_000041.2 and NP_446464.1)

Gene ID: 445, 9606

UniProt: P00966

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin