SMN1 / SMN2 (all Isoforms), (Internal Region) antibody

Catalog No. ABIN374850

This anti- antibody is a Goat Polyclonal antibody detecting in WB. Suitable for Human.

Quick Overview for SMN1 / SMN2 (all Isoforms), (Internal Region) antibody (ABIN374850)

Target: SMN1 / SMN2

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: all Isoforms, Internal Region

Sequence: C-DESENSRSPG NKSDN

Specificity: This antibody is expected to recognise isoforms b and d of SMN1 (NP_075012.1 and NP_000335.1) and all reported isoforms of SMN2 (NP_075013.1, NP_075014.1, NP_075015.1 and NP_059107.1).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic peptide corresponding to internal region of Human SMN1+SMN2 according to NP_075012.1, NP_000335.1, NP_075013.1, NP_075014.1, NP_075015.1, NP_059107.1.

Application Details

Application Notes: Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.3-1 μg/mL. Approx 26 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 28.6 kDa according to NP_075012.1 and NP_075014.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: SMN1 / SMN2

Background: The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly.Synonyms: Component of gems 1, Gemin-1, Gemin1, SMN, SMNC, SMNT, Survival motor neuron protein

Gene ID: 6606

NCBI Accession: NP_000335

UniProt: Q16637