EGLN3 antibody (Internal Region)

Catalog No. ABIN374996

The Goat Polyclonal anti-EGLN3 antibody is suitable to detect EGLN3 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB and EIA.

Quick Overview for EGLN3 antibody (Internal Region) (ABIN374996)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This EGLN3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-EGLN3 Antibody

Binding Specificity: Internal Region

Specificity: This antibody recognizes EGLN3.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-RLGKYYVKERSK, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Alternative Name: EGLN3 / PHD3

Background: PHD3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. It promotes cell death through a caspase-dependent mechanism.Synonyms: Egl nine homolog 3, HIF PH3, HIF-PH3, HIF-prolyl hydroxylase 3, HPH-1, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 3, Prolyl hydroxylase domain-containing protein 3

Gene ID: 112399

NCBI Accession: NP_071356

UniProt: Q9H6Z9

Pathways: Positive Regulation of Endopeptidase Activity