LY6C + LY6G antibody (Cy5)

Catalog No. ABIN375383

The Rat Monoclonal anti- antibody has been validated for FACS. It is suitable to detect in samples from Mouse.

Quick Overview for LY6C + LY6G antibody (Cy5) (ABIN375383)

Target: LY6C + LY6G

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Cy5

Application: Flow Cytometry (FACS)

Clone: RB6-8C5

Product Details

Specificity: Mouse Ly-6G/Ly-6C, Mr 21-25 kDa

Characteristics: Rat Anti-Mouse Ly-6G/Ly-6C-CY5

Purification: Purified

Immunogen: Unknown

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Depletion - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells SPRD, APC, CY5, PE/CY5.5, PE/CY7, APC/CY7, and AF700 0.03 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro complement-dependent cytotoxicity assays, In vivo depletion of Ly-6G+ cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.1 mg in 0.2 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: LY6C + LY6G

Alternative Name: Ly-6G/Ly-6C

Background: Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. 1 In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. 2 In the periphery, Ly-6G is expressed predominantly on neutrophils.