CD86 antibody (Biotin)

Catalog No. ABIN375428

The Rat Monoclonal anti-CD86 antibody has been validated for FACS. It is suitable to detect CD86 in samples from Mouse.

Quick Overview for CD86 antibody (Biotin) (ABIN375428)

Target: CD86

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD86 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 2D10

Product Details anti-CD86 Antibody (Biotin)

Specificity: Mouse CD86, Mr 80 kDa

Characteristics: Rat Anti-Mouse CD86-BIOT

Purification: Purified

Immunogen: Mouse B cell lymphoma cell line 5C2

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Sep - Reported in literature , CMCD - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE, APC, SPRD, and PE/CY7 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vivo and in vitro blocking assays, Identification of CD86+ cells in frozen section

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: CD86/B7-2 is an 80 kDa type I integral membrane protein and a member of the immunoglobulin superfamily of cell surface receptors. Along with CD80/B7-1, CD86 participates in T cell costimulation via interaction with CD28 and CD152/CTLA-4. It is expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells, monocytes and dendritic cells. Since CD86/B7-2 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation