CD19 antibody (APC-Cy7)

Catalog No. ABIN375857

The Mouse Monoclonal anti-CD19 antibody has been validated for FACS. It is suitable to detect CD19 in samples from Mouse.

Quick Overview for CD19 antibody (APC-Cy7) (ABIN375857)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is conjugated to APC-Cy7

Application: Flow Cytometry (FACS)

Clone: MB19-1

Product Details anti-CD19 Antibody (APC-Cy7)

Specificity: Mouse CD19, Mr 95 kDa

Characteristics: Mouse Anti-Mouse CD19-APC/CY7

Purification: Purified

Immunogen: CD19+ mouse pre-B cell line 300.19

Isotype: IgA

Application Details

Application Notes:

• Applications: FC - Quality tested , IP - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells APC/CY5.5 and APC/CY7 conjugates 0.3 g/106 cells PE, APC, SPRD, and PE/CY5.5 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e., B220 + /CD43 + /HSA + ) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM + cells of both B-1 and B-2 lineages are CD19 +, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway