Goat anti-Mouse IgM (Heavy Chain) Antibody (Biotin)

Catalog No. ABIN375906

Quick Overview for Goat anti-Mouse IgM (Heavy Chain) Antibody (Biotin) (ABIN375906)

Target: IgM

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry.

Characteristics: Source: Pooled antisera from goats hyperimmunized with mouse IgM paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.

Purification: Affinity chromatography on pooled human Igs with light chains covalently linked to agarose

Application Details

Application Notes: Working Dilution:
<= 1 µg/10^6 cells or 1:5,000-1:20,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid cycles of freezing and thawing.

Storage: 4 °C

References

Andersson, Dahlgren: "2-Hydroxyethyl methacrylate (HEMA) promotes IgG but not IgM antibody production in vivo in mice." in: European journal of oral sciences, Vol. 119, Issue 4, pp. 305-9, (2011) (PubMed).

Palmer, Stasko, Waters, Thacker: "Examination of the reticular epithelium of the bovine pharyngeal tonsil." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 294, Issue 11, pp. 1939-50, (2011) (PubMed).

Target Details

Target: IgM

Target Type: Antibody