CD3 epsilon antibody (Cy5)
Quick Overview for CD3 epsilon antibody (Cy5) (ABIN376051)
Target
See all CD3 epsilon (CD3E) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Specificity
- Mouse CD3 Mr 25 kDa
-
Characteristics
- Hamster Anti-Mouse CD3e-CY5
-
Purification
- Purified
-
Immunogen
- Immune complexes isolated from C6VL-BS thymoma cells
-
Isotype
- IgG
-
-
-
-
Application Notes
-
- Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Activ - Reported in literature
- Working Dilutions: Flow Cytometry FITC conjugate 3 g/106 cells BIOT conjugate 1 g/106 cells PE, APC, and APC/CY5.5 conjugates 0.3 g/106 cells SPRD and CY5 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 l
-
Comment
-
In vitro activation of T cells
-
Sample Volume
- 1 mL
-
Restrictions
- For Research Use only
-
-
-
Concentration
- 0.1 mg/mL
-
Buffer
- 0.1 mg in 1.0 mL of PBS/Sodium azide
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handling Advice
-
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. -
Storage
- 4 °C
-
Storage Comment
- Store at 2-8°C
-
-
- CD3 epsilon (CD3E)
-
Alternative Name
- CD3e
-
Background
- CD3 ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 ε is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.
-
Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
Target
-