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CD3 epsilon antibody (Cy5)

This Hamster Monoclonal antibody specifically detects CD3 epsilon in FACS. It exhibits reactivity toward Mouse.
Catalog No. ABIN376051

Quick Overview for CD3 epsilon antibody (Cy5) (ABIN376051)

Target

See all CD3 epsilon (CD3E) Antibodies
CD3 epsilon (CD3E)

Reactivity

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Mouse

Host

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Hamster

Clonality

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Monoclonal

Conjugate

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This CD3 epsilon antibody is conjugated to Cy5

Application

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Flow Cytometry (FACS)

Clone

500-A2
  • Specificity

    Mouse CD3 Mr 25 kDa

    Characteristics

    Hamster Anti-Mouse CD3e-CY5

    Purification

    Purified

    Immunogen

    Immune complexes isolated from C6VL-BS thymoma cells

    Isotype

    IgG
  • Application Notes

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Activ - Reported in literature
    • Working Dilutions: Flow Cytometry FITC conjugate 3 g/106 cells BIOT conjugate 1 g/106 cells PE, APC, and APC/CY5.5 conjugates 0.3 g/106 cells SPRD and CY5 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 l

    Comment

    In vitro activation of T cells

    Sample Volume

    1 mL

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    0.1 mg in 1.0 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Target

    CD3 epsilon (CD3E)

    Alternative Name

    CD3e

    Background

    CD3 ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 ε is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.

    Pathways

    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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