CD3 epsilon antibody (Cy5)

Catalog No. ABIN376051

This Hamster Monoclonal antibody specifically detects CD3 epsilon in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD3 epsilon antibody (Cy5) (ABIN376051)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to Cy5

Application: Flow Cytometry (FACS)

Clone: 500-A2

Product Details anti-CD3 epsilon Antibody (Cy5)

Specificity: Mouse CD3 Mr 25 kDa

Characteristics: Hamster Anti-Mouse CD3e-CY5

Purification: Purified

Immunogen: Immune complexes isolated from C6VL-BS thymoma cells

Isotype: IgG

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC conjugate 3 g/106 cells BIOT conjugate 1 g/106 cells PE, APC, and APC/CY5.5 conjugates 0.3 g/106 cells SPRD and CY5 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 l

Comment:

In vitro activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: CD3 ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 ε is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway