Goat anti-Mouse IgA Antibody (PE)

Catalog No. ABIN4369384

Quick Overview for Goat anti-Mouse IgA Antibody (PE) (ABIN4369384)

Target: IgA

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of mouse IgA

Characteristics: Goat Anti-Mouse IgA-PE

Purification: Purification Method: Affinity chromatography on mouse IgA covalently linked to agarose.

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB , Sep , Depletion
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Sundaravinayagam, Rahjouei, Andreani, Tupiņa, Balasubramanian, Saha, Delgado-Benito, Coralluzzo, Daumke, Di Virgilio: "53BP1 Supports Immunoglobulin Class Switch Recombination Independently of Its DNA Double-Strand Break End Protection Function." in: Cell reports, Vol. 28, Issue 6, pp. 1389-1399.e6, (2019) (PubMed).

Delgado-Benito, Rosen, Wang, Gazumyan, Pai, Oliveira, Sundaravinayagam, Zhang, Andreani, Keller, Kieffer-Kwon, Pękowska, Jung, Driesner, Subbotin, Casellas, Chait, Nussenzweig, Di Virgilio: "The Chromatin Reader ZMYND8 Regulates Igh Enhancers to Promote Immunoglobulin Class Switch Recombination." in: Molecular cell, Vol. 72, Issue 4, pp. 636-649.e8, (2019) (PubMed).

Li, Irrazabal, So, Berru, Du, Lam, Ling, Gommerman, Pan-Hammarström, Martin: "The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining." in: Nature communications, Vol. 9, Issue 1, pp. 1006, (2018) (PubMed).

Target Details

Target: IgA

Target Type: Antibody