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CD8 alpha antibody (SPRD)

CD8A Reactivity: Pig FACS Host: Mouse Monoclonal 76-2-11 SPRD
Catalog No. ABIN376725
  • Target See all CD8 alpha (CD8A) Antibodies
    CD8 alpha (CD8A) (CD8a Molecule (CD8A))
    Reactivity
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    Pig
    Host
    • 150
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    • 96
    Mouse
    Clonality
    • 268
    • 97
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    Monoclonal
    Conjugate
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    This CD8 alpha antibody is conjugated to SPRD
    Application
    • 295
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    Flow Cytometry (FACS)
    Brand
    Spectral Red ™
    Specificity
    Porcine CD8α, Mr 35 kDa
    Characteristics
    Mouse Anti-Porcine CD8a-SPRD
    Purification
    Purified
    Immunogen
    Fresh dd miniature swine thymocytes
    Clone
    76-2-11
    Isotype
    IgG2a
    Top Product
    Discover our top product CD8A Primary Antibody
  • Application Notes
    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Comment

    Cytotoxicity assays, In vivo depletion of CD8+ cells

    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Twitchell, Tin, Wen, Zhang, Becker-Dreps, Azcarate-Peril, Vilchez, Li, Ramesh, Weiss, Lei, Bui, Yang, Schultz-Cherry, Yuan: "Modeling human enteric dysbiosis and rotavirus immunity in gnotobiotic pigs." in: Gut pathogens, Vol. 8, pp. 51, (2016) (PubMed).

  • Target
    CD8 alpha (CD8A) (CD8a Molecule (CD8A))
    Alternative Name
    CD8a (CD8A Products)
    Background
    Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 αα homodimer or a CD8 αβ heterodimer. It is found on most thymocytes, and on the 'suppressor/cytotoxic' subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition.
    Pathways
    TCR Signaling
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