CD8 alpha antibody (SPRD)

Catalog No. ABIN376725

This anti-CD8 alpha antibody is a Mouse Monoclonal antibody detecting CD8 alpha in FACS. Suitable for Pig. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD8 alpha antibody (SPRD) (ABIN376725)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to SPRD

Application: Flow Cytometry (FACS)

Clone: 76-2-11

Product Details anti-CD8 alpha Antibody (SPRD)

Brand: Spectral Red ™

Specificity: Porcine CD8α, Mr 35 kDa

Characteristics: Mouse Anti-Porcine CD8a-SPRD

Purification: Purified

Immunogen: Fresh dd miniature swine thymocytes

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Cytotoxicity assays, In vivo depletion of CD8+ cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD8 alpha antibody (SPRD) (ABIN376725)

Twitchell, Tin, Wen, Zhang, Becker-Dreps, Azcarate-Peril, Vilchez, Li, Ramesh, Weiss, Lei, Bui, Yang, Schultz-Cherry, Yuan: "Modeling human enteric dysbiosis and rotavirus immunity in gnotobiotic pigs." in: Gut pathogens, Vol. 8, pp. 51, (2016) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 αα homodimer or a CD8 αβ heterodimer. It is found on most thymocytes, and on the 'suppressor/cytotoxic' subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition.

Pathways: TCR Signaling