CD3 epsilon antibody

Catalog No. ABIN376890

This anti-CD3 epsilon antibody is a Hamster Monoclonal antibody detecting CD3 epsilon in FACS. Suitable for Mouse.

Quick Overview for CD3 epsilon antibody (ABIN376890)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 500-A2

Product Details anti-CD3 epsilon Antibody

Specificity: Mouse CD3 Mr 25 kDa

Characteristics: Hamster Anti-Mouse CD3e-UNLB

Purification: Purified

Immunogen: Immune complexes isolated from C6VL-BS thymoma cells

Isotype: IgG

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC conjugate 3 g/106 cells BIOT conjugate 1 g/106 cells PE, APC, and APC/CY5.5 conjugates 0.3 g/106 cells SPRD and CY5 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 l

Comment:

In vitro activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: CD3 ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 ε is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway