Mouse anti-Rat IgG1 (Fc Region) Antibody

Catalog No. ABIN376920

Quick Overview for Mouse anti-Rat IgG1 (Fc Region) Antibody (ABIN376920)

Target: IgG1

Clonality: Monoclonal

Application: ELISA, Flow Cytometry (FACS)

Clone: G11C5

Isotype: IgG2b

Product Details

Binding Specificity: Fc Region

Reactivity: Rat

Host: Mouse

Specificity: Reacts with the gamma 1 heavy chain (Fc) of Rat IgG1,

Characteristics: Mouse Anti-Rat IgG1-UNLB

Purification: Purified

Immunogen: Rat IgG1 hybridoma

Application Details

Application Notes:

• Applications: FC - Quality tested ELISA - Quality tested FLISA - Quality tested IHC-FS - Reported in literature , ELISPOT - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, CY5, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:100 - 1:400 PE, CY5, and AF647 conjugates 1 g/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added, store at 2-8 °C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: IgG1

Target Type: Antibody