Transferrin Receptor antibody (Biotin)

Catalog No. ABIN380057

The Rat Monoclonal anti-Transferrin Receptor antibody has been validated for IHC and IHC (fro). It is suitable to detect Transferrin Receptor in samples from Mouse.

Quick Overview for Transferrin Receptor antibody (Biotin) (ABIN380057)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ER-MP21

Product Details anti-Transferrin Receptor Antibody (Biotin)

Specificity: The antigen is a 200  kDa protein (non-reducing conditions) consisting of two identical chains. The recognized epitope of the receptor is not located at the transferrin binding site. Antigen distribution: Differentiation stage-dependent inhibition of macrophage proliferation by ER-MP21 in MCSF-stimulated bone marrow cultures. Macrophage progeny at day 7 in bone marrow cultures, treated in parallel on various days by irradiation (1500 rad 60Co gamma-radiation) or addition of ER-MP21 (30 μg/mL final concentration). Cell numbers were quantified using the MTT assay and expressed relative to untreated controls. Relative growth inhibition calculated from data represented in left figure. For mAbor radiation treated cultures, the number of cell cycles needed to reach the cell number in untreated controls was calculated. Values obtained for irradiated cultures were taken as 100 % inhibition controls and values for ER-MP21 - treated cultures were expressed relative to these. Specificity: Mouse: transfer in receptor. Other species: not tested.

Purification: Affinity purified

Immunogen: Macrophage precursor cells

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:200)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 10 mg/mL BSA, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / Transferrin Receptor

Background: Name/Gene ID: TFRC
Subfamily: Metallopeptidase M28B
Family: Protease

Synonyms: TFRC, CD71, TFR1, TRFR, TFR, TR, CD71 antigen, p90, T9, Transferrin receptor, Transferrin receptor protein 1

Gene ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis