beta Actin antibody (HRP)

Catalog No. ABIN7477941

This anti-beta Actin antibody is a Mouse Monoclonal antibody detecting beta Actin in WB and ELISA. Suitable for Human, Mouse, Rat, Chicken, Rabbit, Pig, Cow, Hamster, Goat and Fish. This Primary Antibody has been cited in 3+ publications.

Quick Overview for beta Actin antibody (HRP) (ABIN7477941)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Clone: 2D1D10

Product Details anti-beta Actin Antibody (HRP)

Specificity: Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig substrates. It has not yet been tested in other species.

Cross-Reactivity (Details): Cross reactivity in other species has not yet been tested.

Characteristics: This product provides a useful and serves as a specific tool in the study of the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Do not add azide because it inhibits the activity of HRP.

Purification: Protein G affinity column

Immunogen: A synthetic peptide DDDIAALVVDNGSG coupled - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References for anti-beta Actin antibody (HRP) (ABIN7477941)

Ferreira, Wang: "Optimization of oncogene expression through intra-population competition." in: Biotechnology journal, Vol. 8, Issue 12, pp. 1476-84, (2013) (PubMed).

Jiménez-Soto, Clausen, Sprenger, Ertl, Haas: "Dynamics of the Cag-type IV secretion system of Helicobacter pylori as studied by bacterial co-infections." in: Cellular microbiology, Vol. 15, Issue 11, pp. 1924-37, (2013) (PubMed).

Berry, Shin, Lightfoot, Janknecht: "Oncogenic features of the JMJD2A histone demethylase in breast cancer." in: International journal of oncology, Vol. 41, Issue 5, pp. 1701-6, (2012) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta Actin

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction