DYKDDDDK Tag antibody (Biotin)

Catalog No. ABIN387716

The Mouse Monoclonal anti-DYKDDDDK Tag antibody is suitable to detect DYKDDDDK Tag. It has been validated for WB and ELISA.

Quick Overview for DYKDDDDK Tag antibody (Biotin) (ABIN387716)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: 5A8E5

Product Details anti-DYKDDDDK Tag Antibody (Biotin)

Sequence: DYKDDDDK

Specificity: THE^TM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged fusion proteins.

Characteristics: The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins.

Purification: Protein A affinity column

Immunogen: A synthetic peptide (DYKDDDDK) coupled - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Target Type: Tag