GFP Tag antibody

Catalog No. ABIN387749

The Mouse Monoclonal anti-GFP Tag antibody has been validated for WB. It is suitable to detect GFP Tag in samples from . There are 2+ publications available.

Quick Overview for GFP Tag antibody (ABIN387749)

Target: GFP Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP Tag antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 168AT1211-269-64

Product Details anti-GFP Tag Antibody

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogen: Purified His-tagged GFP protein was used to produced this monoclonal antibody.

Isotype: IgG

Application Details

Application Notes: WB: 1:100~500. WB: 1:100~500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-GFP Tag antibody (ABIN387749)

Netsu, Hiraguri, Uehara-Ichiki, Komatsu, Sasaya: "Functional comparison of RNA silencing suppressor between the p5 protein of rice grassy stunt virus and the p3 protein of rice stripe virus." in: Virus research, Vol. 203, pp. 10-9, (2015) (PubMed).

Nicholls, Chu, Abbruzzese, Tremblay, Hardy: "Mechanism of a genetically encoded dark-to-bright reporter for caspase activity." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24977-86, (2011) (PubMed).

Target Details for GFP Tag

Target: GFP Tag

Target Type: Tag

Background: Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.