GST-Tag antibody

Catalog No. ABIN387751

This Mouse Monoclonal antibody specifically detects GST-Tag in WB. It exhibits reactivity toward and has been mentioned in 6+ publications.

Quick Overview for GST-Tag antibody (ABIN387751)

Target: GST-Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This GST-Tag antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 9AT106

Product Details anti-GST-Tag Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified recombinant GST fusion protein was used to produced this monoclonal antibody.

Isotype: IgG1

Application Details

Application Notes: WB: 1:2,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Expiry Date: 6 months

References for anti-GST-Tag antibody (ABIN387751)

Messina, Atterrato, Fanti, Giordano, Dimitri: "Expression of human Cfdp1 gene in Drosophila reveals new insights into the function of the evolutionarily conserved BCNT protein family." in: Scientific reports, Vol. 6, pp. 25511, (2018) (PubMed).

Zhong, Li, Cai, Wang, Dong, Yang, Zhang, Wu, Li, Mao, Zeng, Wu, Xu, Sun: "TET1 modulates H4K16 acetylation by controlling auto-acetylation of hMOF to affect gene regulation and DNA repair function." in: Nucleic acids research, Vol. 45, Issue 2, pp. 672-684, (2017) (PubMed).

Fu, Olsen, Webb, Bennett, Lykke-Andersen: "Recruitment of the 4EHP-GYF2 cap-binding complex to tetraproline motifs of tristetraprolin promotes repression and degradation of mRNAs with AU-rich elements." in: RNA (New York, N.Y.), Vol. 22, Issue 3, pp. 373-82, (2016) (PubMed).

Itagaki, Naito, Iwakiri, Haga, Miura, Saito, Owaki, Kamiya, Iyoda, Yajima, Iwashita, Ejiri, Fukai: "Eukaryotic translation elongation factor 1A induces anoikis by triggering cell detachment." in: The Journal of biological chemistry, Vol. 287, Issue 19, pp. 16037-46, (2012) (PubMed).

Wang, Fan, Williamson, Qin: "Intraflagellar transport (IFT) protein IFT25 is a phosphoprotein component of IFT complex B and physically interacts with IFT27 in Chlamydomonas." in: PLoS ONE, Vol. 4, Issue 5, pp. e5384, (2009) (PubMed).

Nery, Zeng, Niland, Hewett, Farley, Irimia, Li, Wiche, Sonnenberg, Breakefield: "TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton." in: Journal of cell science, Vol. 121, Issue Pt 20, pp. 3476-86, (2008) (PubMed).

Target Details for GST-Tag

Target: GST-Tag

Alternative Name: GST Tag

Target Type: Tag

Background: Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.

Molecular Weight: 26000