Nanog antibody

Catalog No. ABIN387791

The Mouse Monoclonal anti-Nanog antibody has been validated for WB and IHC (p). It is suitable to detect Nanog in samples from Human.

Quick Overview for Nanog antibody (ABIN387791)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 60CT77-1-1

Product Details anti-Nanog Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:100~250. WB: 1:100~250. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).

Molecular Weight: 34620

Gene ID: 79923

NCBI Accession: NP_079141

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance