UCHL5 antibody (N-Term)

Catalog No. ABIN388873

This Rabbit Polyclonal antibody specifically detects UCHL5 in WB and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for UCHL5 antibody (N-Term) (ABIN388873)

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RB4190

Product Details anti-UCHL5 Antibody

Binding Specificity: AA 56-87, N-Term

Predicted Reactivity: B, Pig

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This UCH37 (UCHL5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-87 amino acids from the N-terminal region of human UCH37 (UCHL5).

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-F: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for UCHL5

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Alternative Name: UCH37 (UCHL5)

Background: Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.

Molecular Weight: 37607

Gene ID: 51377

NCBI Accession: NP_001186190, NP_001186191, NP_001186192, NP_057068

UniProt: Q9Y5K5