USP3 antibody (N-Term)

Catalog No. ABIN388880

The Rabbit Polyclonal anti-USP3 antibody has been validated for WB and IHC (p). It is suitable to detect USP3 in samples from Human.

Quick Overview for USP3 antibody (N-Term) (ABIN388880)

Target: USP3 (Ubiquitin Specific Peptidase 3 (USP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB4205-4206

Product Details anti-USP3 Antibody

Binding Specificity: AA 114-143, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This USP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-143 amino acids from the N-terminal region of human USP3.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for USP3

Target: USP3 (Ubiquitin Specific Peptidase 3 (USP3))

Alternative Name: USP3

Background: Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Molecular Weight: 58897

Gene ID: 9960

NCBI Accession: NP_001243631, NP_006528

UniProt: Q9Y6I4