UBE2E3 antibody (N-Term)

Catalog No. ABIN388952

The Rabbit Polyclonal anti-UBE2E3 antibody has been validated for WB and IHC (p). It is suitable to detect UBE2E3 in samples from Human and Mouse.

Quick Overview for UBE2E3 antibody (N-Term) (ABIN388952)

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2E3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB4431

Product Details anti-UBE2E3 Antibody

Binding Specificity: AA 10-40, N-Term

Predicted Reactivity: B

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This UBCH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-40 amino acids from the N-terminal region of human UBCH9.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for UBE2E3

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Alternative Name: UBCH9

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBCH9 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100 % sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found.

Molecular Weight: 22913

Gene ID: 10477

NCBI Accession: NP_001265483, NP_001265484, NP_006348, NP_872619

UniProt: Q969T4