APRT antibody (C-Term)

Catalog No. ABIN389427

This anti-APRT antibody is a Rabbit Polyclonal antibody detecting APRT in WB and IHC (p). Suitable for Human and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for APRT antibody (C-Term) (ABIN389427)

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APRT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB20510

Product Details anti-APRT Antibody

Binding Specificity: AA 143-170, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-170 amino acids from the C-terminal region of human APRT.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-APRT antibody (ABIN389427)

Valaperta, Rizzo, Lombardi, Verdelli, Piccoli, Ghiroldi, Creo, Colombo, Valisi, Margiotta, Panella, Costa: "Adenine phosphoribosyltransferase (APRT) deficiency: identification of a novel nonsense mutation." in: BMC nephrology, Vol. 15, pp. 102, (2014) (PubMed).

Target Details for APRT

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Alternative Name: APRT

Background: A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

Molecular Weight: 19608

Gene ID: 353

NCBI Accession: NP_000476, NP_001025189

UniProt: P07741

Pathways: Ribonucleoside Biosynthetic Process