CRY1 antibody (C-Term)

Catalog No. ABIN390079

This Rabbit Polyclonal antibody specifically detects CRY1 in WB and IHC (p). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for CRY1 antibody (C-Term) (ABIN390079)

Target: CRY1 (Cryptochrome 1 (Photolyase-Like) (CRY1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1844

Product Details anti-CRY1 Antibody

Binding Specificity: AA 556-586, C-Term

Predicted Reactivity: Pr

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Cry1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 556-586 amino acids from the C-terminal region of human Cry1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-CRY1 antibody (ABIN390079)

Wang, Wang, Liu, Liu, Tay, Walsh, Yang, Wu: "CRISPR/Cas9 mediated genome editing of Helicoverpa armigera with mutations of an ABC transporter gene HaABCA2 confers resistance to Bacillus thuringiensis Cry2A toxins." in: Insect biochemistry and molecular biology, Vol. 87, pp. 147-153, (2017) (PubMed).

Target Details for CRY1

Target: CRY1 (Cryptochrome 1 (Photolyase-Like) (CRY1))

Alternative Name: Cry1

Background: Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central ?gears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function, in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Molecular Weight: 66395

Gene ID: 1407

NCBI Accession: NP_004066

UniProt: Q16526

Pathways: Response to Water Deprivation, Proton Transport