NDUFS4 antibody (C-Term)

Catalog No. ABIN390919

This Rabbit Polyclonal antibody specifically detects NDUFS4 in WB, FACS and IHC (p). It exhibits reactivity toward Human and Mouse.It has been mentioned in 1 publication

Quick Overview for NDUFS4 antibody (C-Term) (ABIN390919)

Target: NDUFS4 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 4, 18kDa (NADH-Coenzyme Q Reductase) (NDUFS4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDUFS4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB21548

Product Details anti-NDUFS4 Antibody

Binding Specificity: AA 131-160, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NDUFS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-160 amino acids from the C-terminal region of human NDUFS4.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P-Leica: 1:250. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-NDUFS4 antibody (ABIN390919)

Boominathan, Vanhoozer, Basisty, Powers, Crampton, Wang, Friedricks, Schilling, Brand, OConnor: "Stable nuclear expression of ATP8 and ATP6 genes rescues a mtDNA Complex V null mutant." in: Nucleic acids research, Vol. 44, Issue 19, pp. 9342-9357, (2016) (PubMed).

Target Details for NDUFS4

Target: NDUFS4 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 4, 18kDa (NADH-Coenzyme Q Reductase) (NDUFS4))

Alternative Name: NDUFS4

Background: NDUFS4 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase(Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane.

Molecular Weight: 20108

Gene ID: 4724

NCBI Accession: NP_002486

UniProt: O43181