PNCK antibody (C-Term)

Catalog No. ABIN391139

There are 2+ publications for this product available. The Rabbit Polyclonal anti-PNCK antibody is suitable to detect PNCK in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for PNCK antibody (C-Term) (ABIN391139)

Target: PNCK (Pregnancy Up-Regulated Non-Ubiquitously Expressed CaM Kinase (PNCK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1253-1254

Product Details anti-PNCK Antibody

Binding Specificity: AA 314-343, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PNCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the C-terminal region of human PNCK.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PNCK antibody (ABIN391139)

Deb, Zuo, Wang, Barndt, Cheema, Sengupta, Coticchia, Johnson: "Pnck induces ligand-independent EGFR degradation by probable perturbation of the Hsp90 chaperone complex." in: American journal of physiology. Cell physiology, Vol. 300, Issue 5, pp. C1139-54, (2011) (PubMed).

Deb, Coticchia, Barndt, Zuo, Dickson, Johnson: "Pregnancy-upregulated nonubiquitous calmodulin kinase induces ligand-independent EGFR degradation." in: American journal of physiology. Cell physiology, Vol. 295, Issue 2, pp. C365-77, (2008) (PubMed).

Target Details for PNCK

Target: PNCK (Pregnancy Up-Regulated Non-Ubiquitously Expressed CaM Kinase (PNCK))

Alternative Name: PNCK

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Molecular Weight: 38500

Gene ID: 139728

NCBI Accession: NP_001034671, NP_001129212

UniProt: Q6P2M8