GCAT antibody (AA 155-181)

Catalog No. ABIN391717

This Rabbit Polyclonal antibody specifically detects GCAT in WB, FACS and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for GCAT antibody (AA 155-181) (ABIN391717)

Target: GCAT (Glycine C-Acetyltransferase (GCAT))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCAT antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB18219

Product Details anti-GCAT Antibody

Binding Specificity: AA 155-181

Predicted Reactivity: B

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for GCAT

Target: GCAT (Glycine C-Acetyltransferase (GCAT))

Alternative Name: GCAT

Background: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.

Molecular Weight: 45285

Gene ID: 23464

NCBI Accession: NP_001165161, NP_055106

UniProt: O75600