BACE1 antibody (N-Term)

Catalog No. ABIN392201

The Rabbit Polyclonal anti-BACE1 antibody has been validated for WB and IHC (p). It is suitable to detect BACE1 in samples from Human and Mouse. There are 4+ publications available.

Quick Overview for BACE1 antibody (N-Term) (ABIN392201)

Target: BACE1 (Beta-secretase 1 (BACE1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BACE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB16874

Product Details anti-BACE1 Antibody

Binding Specificity: AA 38-70, N-Term

Predicted Reactivity: B, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This BACE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-70 amino acids from the N-terminal region of human BACE1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-BACE1 antibody (ABIN392201)

Lu, Cui, Li, Wang, Zhou, Yang, Chen, Zhao, Pei: "An Anti-Parkinson's Disease Drug via Targeting Adenosine A2A Receptor Enhances Amyloid-β Generation and γ-Secretase Activity." in: PLoS ONE, Vol. 11, Issue 11, pp. e0166415, (2017) (PubMed).

Lu, Li, Wang, Pei: "Dopamine D2 receptor and β-arrestin 2 mediate Amyloid-β elevation induced by anti-parkinson's disease drugs, levodopa and piribedil, in neuronal cells." in: PLoS ONE, Vol. 12, Issue 3, pp. e0173240, (2017) (PubMed).

Li, Cui, Yu, Li, Hou, Wang, Qin, Zhao, Yao, Zhao, Pei: "Traditional Chinese Nootropic Medicine Radix Polygalae and Its Active Constituent Onjisaponin B Reduce β-Amyloid Production and Improve Cognitive Impairments." in: PLoS ONE, Vol. 11, Issue 3, pp. e0151147, (2016) (PubMed).

Nie, Li, Yan, Wang, Zhao, Guo, Yin: "Nicotine decreases beta-amyloid through regulating BACE1 transcription in SH-EP1-?4?2 nAChR-APP695 cells." in: Neurochemical research, Vol. 36, Issue 5, pp. 904-12, (2011) (PubMed).

Target Details for BACE1

Target: BACE1 (Beta-secretase 1 (BACE1))

Alternative Name: BACE1

Background: Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is BACE. This protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi.

Molecular Weight: 55764

Gene ID: 23621

NCBI Accession: NP_001193978, NP_036236, NP_620427, NP_620428, NP_620429

UniProt: P56817