STK39 antibody (AA 346-376)

Catalog No. ABIN392497

This Rabbit Polyclonal antibody specifically detects STK39 in WB and IHC (p). It exhibits reactivity toward Human and Mouse and has been mentioned in 4+ publications.

Quick Overview for STK39 antibody (AA 346-376) (ABIN392497)

Target: STK39 (serine threonine Kinase 39 (STK39))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STK39 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB03418

Product Details anti-STK39 Antibody

Binding Specificity: AA 346-376

Predicted Reactivity: Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SPAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-STK39 antibody (ABIN392497)

Yang, Cai, Zhou, Chen, Chen, Chen, Wang, Fang, Zhou: "STE20/SPS1-related proline/alanine-rich kinase is involved in plasticity of GABA signaling function in a mouse model of acquired epilepsy." in: PLoS ONE, Vol. 8, Issue 9, pp. e74614, (2013) (PubMed).

Wang, OConnell, McArdle, Wade, Dorff, Shah, Shi, Pan, Rampersaud, Shen, Kim, Subramanya, Steinle, Parsa, Ober, Welling, Chakravarti, Weder, Cooper, Mitchell, Shuldiner, Chang: "From the Cover: Whole-genome association study identifies STK39 as a hypertension susceptibility gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 1, pp. 226-31, (2009) (PubMed).

Smith, Smallwood, Altman, Liedtke: "PKCdelta acts upstream of SPAK in the activation of NKCC1 by hyperosmotic stress in human airway epithelial cells." in: The Journal of biological chemistry, Vol. 283, Issue 32, pp. 22147-56, (2008) (PubMed).

Liedtke, Wang, Smallwood: "Role for protein phosphatase 2A in the regulation of Calu-3 epithelial Na+-K+-2Cl-, type 1 co-transport function." in: The Journal of biological chemistry, Vol. 280, Issue 27, pp. 25491-8, (2005) (PubMed).

Target Details for STK39

Target: STK39 (serine threonine Kinase 39 (STK39))

Alternative Name: SPAK

Background: SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.

Molecular Weight: 59474

Gene ID: 27347

NCBI Accession: NP_037365

UniProt: Q9UEW8