PIK3R3 antibody (C-Term)

Catalog No. ABIN392576

Product Details anti-PIK3R3 Antibody

Target: PIK3R3 (Phosphatidylinositol 3-kinase regulatory subunit gamma (PIK3R3))

Binding Specificity: AA 316-346, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PIK3R3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-346 amino acids from the C-terminal region of human PIK3R3.

Clone: RB1719

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:100. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PIK3R3 antibody (ABIN392576)

Yang, Cheng, Zhou, Zhu, Hu, Yang: "Overexpression of X-Box Binding Protein 1 (XBP1) Correlates to Poor Prognosis and Up-Regulation of PI3K/mTOR in Human Osteosarcoma." in: International journal of molecular sciences, Vol. 16, Issue 12, pp. 28635-46, (2016) (PubMed).

Wang, Yang, Jin, Deng, Luo, Hu, Wang: "TGF-? regulates the proliferation of lung adenocarcinoma cells by inhibiting PIK3R3 expression." in: Molecular carcinogenesis, (2014) (PubMed).

Eiring, Neviani, Santhanam, Oaks, Chang, Notari, Willis, Gambacorti-Passerini, Volinia, Marcucci, Caligiuri, Leone, Perrotti: "Identification of novel posttranscriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis." in: Blood, Vol. 111, Issue 2, pp. 816-28, (2008) (PubMed).

Zhang, Huang, Yang, Greshock, Liang, Hasegawa, Giannakakis, Poulos, OBrien-Jenkins, Katsaros, Butzow, Weber, Coukos: "Integrative genomic analysis of phosphatidylinositol 3'-kinase family identifies PIK3R3 as a potential therapeutic target in epithelial ovarian cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 18 Pt 1, pp. 5314-21, (2007) (PubMed).

Target Details for PIK3R3

Target: PIK3R3 (Phosphatidylinositol 3-kinase regulatory subunit gamma (PIK3R3))

Alternative Name: PIK3R3 (PIK3R3 Products)

Background: PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.

Molecular Weight: 54448

Gene ID: 8503

NCBI Accession: NP_001107644, NP_003620

UniProt: Q92569