SIK1 antibody (C-Term)

Catalog No. ABIN392683

This Rabbit Polyclonal antibody specifically detects SIK1 in WB and IHC (p). It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for SIK1 antibody (C-Term) (ABIN392683)

Target: SIK1 (Salt-Inducible Kinase 1 (SIK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB3576

Product Details anti-SIK1 Antibody

Binding Specificity: AA 567-597, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SNF1LK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 567-597 amino acids from the C-terminal region of human SNF1LK.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-SIK1 antibody (ABIN392683)

Cheng, Liu, Wang, Zou, Santiago, Garbitt, Gjoerup, Iglehart, Miron, Richardson, Hahn, Zhao: "SIK1 couples LKB1 to p53-dependent anoikis and suppresses metastasis." in: Science signaling, Vol. 2, Issue 80, pp. ra35, (2009) (PubMed).

Target Details for SIK1

Target: SIK1 (Salt-Inducible Kinase 1 (SIK1))

Alternative Name: SNF1LK

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Molecular Weight: 84902

Gene ID: 150094

NCBI Accession: NP_775490

UniProt: P57059

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process