CD3 antibody (APC)

Catalog No. ABIN392945

The Rat Monoclonal anti-CD3 antibody has been validated for FACS, IHC and IHC (fro). It is suitable to detect CD3 in samples from Mouse.

Quick Overview for CD3 antibody (APC) (ABIN392945)

Target: CD3

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: KT3

Product Details anti-CD3 Antibody (APC)

Specificity: Recognizes the mouse CD3 antigen which is expressed by mature T cells. For optimal staining incubations should be performed at room temperature.

Purification: Affinity purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1), IHC, IHC-Fr

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway